normal lung epithelial cell line Search Results


huv-ec-c  (ATCC)
96
ATCC huv-ec-c
Huv Ec C, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/atcc___crl-1730?v=ATCC
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huv-ec-c - by Bioz Stars, 2026-06
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human lung epithelial cell complete medium  (Celprogen Inc)
90
Celprogen Inc human lung epithelial cell complete medium
Human Lung Epithelial Cell Complete Medium, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm24325134-68-13-19?v=Celprogen+Inc
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human lung epithelial cell complete medium - by Bioz Stars, 2026-06
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a549 luc1 stable luciferase-transfected human type ii lung epithelial cell line  (AAALAC International Inc)
90
AAALAC International Inc a549 luc1 stable luciferase-transfected human type ii lung epithelial cell line
A549 Luc1 Stable Luciferase Transfected Human Type Ii Lung Epithelial Cell Line, supplied by AAALAC International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pmc03884809-709-0-11?v=AAALAC+International+Inc
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a549 luc1 stable luciferase-transfected human type ii lung epithelial cell line - by Bioz Stars, 2026-06
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human lung epithelial cell line beas-2b  (ABclonal Biotechnology)
90
ABclonal Biotechnology human lung epithelial cell line beas-2b
Human Lung Epithelial Cell Line Beas 2b, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm38244585-97-1-10?v=ABclonal+Biotechnology
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human lung epithelial cell line beas-2b - by Bioz Stars, 2026-06
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immortalized normal lung epithelial cell line sale 37  (Lonza)
90
Lonza immortalized normal lung epithelial cell line sale 37
Immortalized Normal Lung Epithelial Cell Line Sale 37, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pmc04084668-170-2-16?v=Lonza
Average 90 stars, based on 1 article reviews
immortalized normal lung epithelial cell line sale 37 - by Bioz Stars, 2026-06
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adherent adenocarcinoma human alveolar basal epithelial a459 lung cancer cell line  (National Centre for Cell Science)
90
National Centre for Cell Science adherent adenocarcinoma human alveolar basal epithelial a459 lung cancer cell line
Adherent Adenocarcinoma Human Alveolar Basal Epithelial A459 Lung Cancer Cell Line, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/10__3390_slash_ddc3030033-331-1-16?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
adherent adenocarcinoma human alveolar basal epithelial a459 lung cancer cell line - by Bioz Stars, 2026-06
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t.brasiliensis lung epithelial cell line (bat cell line)  (National Centre for Cell Science)
90
National Centre for Cell Science t.brasiliensis lung epithelial cell line (bat cell line)
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
T.Brasiliensis Lung Epithelial Cell Line (Bat Cell Line), supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pmc11652944-77-0-12?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
t.brasiliensis lung epithelial cell line (bat cell line) - by Bioz Stars, 2026-06
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wi26va4 human normal lung cell fibroblasts  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures wi26va4 human normal lung cell fibroblasts
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Wi26va4 Human Normal Lung Cell Fibroblasts, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm33416428-39-12-27?v=European+Collection+of+Authenticated+Cell+Cultures
Average 90 stars, based on 1 article reviews
wi26va4 human normal lung cell fibroblasts - by Bioz Stars, 2026-06
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lung normal epithelial cell line l123  (National Centre for Cell Science)
90
National Centre for Cell Science lung normal epithelial cell line l123
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Lung Normal Epithelial Cell Line L123, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm27810772-48-5-15?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
lung normal epithelial cell line l123 - by Bioz Stars, 2026-06
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normal lung epithelial cells (nl20)  (National Centre for Cell Science)
90
National Centre for Cell Science normal lung epithelial cells (nl20)
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Normal Lung Epithelial Cells (Nl20), supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm24986452-221-15-24?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
normal lung epithelial cells (nl20) - by Bioz Stars, 2026-06
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human lung adenocarcinoma epithelial a549 cell line  (ImmunoMolecular Therapeutics LLC)
90
ImmunoMolecular Therapeutics LLC human lung adenocarcinoma epithelial a549 cell line
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Human Lung Adenocarcinoma Epithelial A549 Cell Line, supplied by ImmunoMolecular Therapeutics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm36994673-27-3-14?v=ImmunoMolecular+Therapeutics+LLC
Average 90 stars, based on 1 article reviews
human lung adenocarcinoma epithelial a549 cell line - by Bioz Stars, 2026-06
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lung epithelial cell line a549  (TranScrip Partners)
90
TranScrip Partners lung epithelial cell line a549
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Lung Epithelial Cell Line A549, supplied by TranScrip Partners, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+lung+epithelial+cell+line/pm12186826-169-4-9?v=TranScrip+Partners
Average 90 stars, based on 1 article reviews
lung epithelial cell line a549 - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet: Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.

Article Snippet: Tb1 Lu ( T.brasiliensis lung epithelial cell line (bat cell line)) , National Centre for Cell Science (NCCS) cell repository , N/A.

Techniques: RNA Binding Assay, Infection, Expressing, Western Blot, Transduction, Staining, Imaging, Transfection

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet:

Article Snippet: Tb1 Lu ( T.brasiliensis lung epithelial cell line (bat cell line)) , National Centre for Cell Science (NCCS) cell repository , N/A.

Techniques: Virus, Recombinant, Transfection, Staining, Western Blot, Electron Microscopy, Plasmid Preparation, Software, Microscopy