Journal: Oncology Letters

Article Title: Antitumor activity of nimotuzumab in combination with cisplatin in lung cancer cell line A549 in vitro

doi: 10.3892/ol.2018.7923

Figure Lengend Snippet: Cyclin D1 expression in A549 cells in groups A-E following treatment for 48 h. Lanes: A, nimotuzumab; B, cisplatin; C, nimotuzumab followed by cisplatin; D, nimotuzumab and cisplatin simultaneously; and E, untreated control.

Article Snippet: Cell culture The A549 human lung adenocarcinoma epithelial cell line (supplied by the Central Laboratory of Qinhuangdao No. 1 People's Hospital) was cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Zhejiang Tianhang Biotechnology Co., Ltd., Huzhou, China), 1 U/ml penicillin and 1 mg/ml streptomycin at 37°C, with 5% CO 2 and 95% humidity.

Techniques: Expressing, Control

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet: Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.

Article Snippet: Tb1 Lu ( T.brasiliensis lung epithelial cell line (bat cell line)) , National Centre for Cell Science (NCCS) cell repository , N/A.

Techniques: RNA Binding Assay, Infection, Expressing, Western Blot, Transduction, Staining, Imaging, Transfection

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet:

Article Snippet: Tb1 Lu ( T.brasiliensis lung epithelial cell line (bat cell line)) , National Centre for Cell Science (NCCS) cell repository , N/A.

Techniques: Virus, Recombinant, Transfection, Staining, Western Blot, Electron Microscopy, Plasmid Preparation, Software, Microscopy